MCQ on Biotechnology - Plant Tissue Culture. Which of the following can be done in different plant species, 2._____________is the advantage of somatic hybridization over sexual hybridization, b) It can be done in the same plant species, c) It can be done in different plant species, 3. This is one of the better methods as it ensures support for the protoplast. MCQ on Indian Art and Culture PDF Download Link Here I am providing Indian Art and Culture Objective Questions and Replies for all those students that are preparing for competitive exams. Complete Protoplast Culture Botany Notes | EduRev chapter (including extra questions, long questions, short questions, mcq) can be found on EduRev, you can check out Botany lecture & lessons summary in the same course for Botany Syllabus. Cultures of established Cell Lines; NEET MCQ tissue-culture PDF:-Download the PDF Here. It is not always possible to produce plants that give visible or fertile seeds, Genetic instability is associated with protoplast culture, Diploid protoplast is not recommended for protoplasmic fusion. These short solved questions or quizzes are provided by Gkseries. Flashcards. 4. For cell culture applications the standard configuration is N2 as the balance gas and O2 and CO2 as the solute gases but, under customer’s request, the instrument can be set with other desired non-aggressive gas media. Hormone pair required for a protoplast füzyonu ile melezlenebilmeleri, Protoplastlar bünyelerine makro moleküller, organeller ve hatta hücre çekirdekleri de alabildiğinden, istenilen bilgilerin yeni bitkilere aktarılması. MCQ on Indian Art and Culture PDF Download Link Here I am providing Indian Art and Culture Objective Questions and Replies for all those students that are preparing for competitive exams. 9 Which plants have already been bred with the help of protoplast fusion? To date, antibiotic resistant protoplast-derived plants have been obtained, but stable plastid transformation has yet to be confirmed. However, there have been no studies on the isolation and culture of protoplasts from irradiated plants for the selection and regeneration of salt-resistant mutants. culturing is called, 10. 2. MCQ Instruments calibrates its gas mixers with native gases. Your email address will not be published. Optimal density to the culture. 10 Cybrids are hybrids only for cytoplasm. Ace up your preparation with the Objective Questions available on Plant Tissue Culture and enhance your subject knowledge. The results are reported for the first time on successful plant regeneration from mesophyll-derived protoplasts of ‘rapid cycling’ B. oleracea. Factors affecting protoplast culture 4. (c) vegetative multiplication of plants by using microspores. It is difficult to produce viable somatic hybrids by protoplasmic fusion. MCQ quiz on Plant Tissue Culture multiple choice questions and answers on Plant Tissue Culture MCQ questions on Plant Tissue Culture objectives questions with answer test pdf for interview preparations, freshers jobs and competitive exams Page 4 MCQ Questions for Class 12 Biology with Answers were prepared based on the latest exam pattern. The medium should be devoid of ammonium, and the quantities of iron and zinc should be less. I was trying several methods only with commercial enzymes (cellulase R-10 and alginate lyase from Sigma) for isolation protoplast with a final yield between 7000 and 17 000 protoplast… Spell. Micro propagation involves (a) vegetative multiplication of plants by using micro-organisms. The two plant protoplast can be stained with fluorescent dyes to identify the fusion products under a fluorescence microscope. MCQ On MCQ On. Sexual hybridization can be only be used for the same plant species. 1. Who is known as the Father of tissue culture? Practice MCQ Questions on Indian Culture. Kolkata, books & allied (p) ltd publishers, 2005  page: 528-537 and  557-559. Pride C. Anger D. Spiritual Knowledge (X) Answer: Maybe Morality 2. Some of the special features of protoplast culture media are listed below: 1. To date, antibiotic resistant protoplast-derived plants have been obtained, but stable plastid transformation has yet to be confirmed. However, there have been no studies on the isolation and culture of protoplasts from irradiated plants for the selection and regeneration of salt-resistant mutants. It is a cost-effective process, as it requires very little space and the cost of production is very low. Production of pathogen-free plants can be done by micropropagation. Check the below NCERT MCQ Questions for Class 12 Biology Chapter 12 Biotechnology and its Applications with Answers Pdf free download. 3. MCQ quiz on Plant Tissue Culture multiple choice questions and answers on Plant Tissue Culture MCQ questions on Plant Tissue Culture objectives questions with answer test pdf for interview preparations, freshers jobs and competitive exams Page 3 15. Somatic embryos are the same as sexually formed or zygotic embryos, which can be excised from parent tissues and induced to germinate into culture media. This is only a method for in-vivo propagation. The first step in protoplast culture is the development of a cell wall around the membrane of the protoplast. Once the wall is formed, the proto­plast becomes a cell. Home / Class 8 Science / Chapter – 8 Cell – Structure and functions MCQ Test 2 Science | Class – 8th. MCQ on Plant Biotechnology - Plant Tissue Culture. (a) Remains stable at incubation temperature (b) Does not react with media constituents (c) Does not use in micropropagation work (d) Not digested by plant enzymes . To remove PEG, the protoplasts are then washed several times by the addition of protoplast culture medium. Protoplast culture is the aseptic isolation & culture of protoplast in vitro with the obtaining of viable plant. Sequential cell division leads to callus formation. From this callus by organogenetic or embryo-genetic differentiation the plantlets or embryos may develop (Fig. Admission), MCQ for somatic hybridization and somatic embryogensis for CSIR NET, MCQ for somatic hybridization and somatic embryogensis for GPAT, Myxoedema : Definition, Causes, Symptoms, Clinical Features, Treatment And MCQs for NEET, GPAT, CSIR NET JRF, Role of National Education Policy in Achieving UN Sustainable Development Goals: Experts View, https://www.youtube.com/watch?v=KZeejstpwSE&t=23s, https://www.youtube.com/watch?v=gs_jxI2xvUE. Test. Protoplast Fusion. Plant protoplast has provided novel approaches, to create a new genetically modified cell. In Somatic hybridization, a hybrid cell is formed by In-vitro protoplast fusion. c) Somatic hybridization. c) Nucleus of one species but cytoplasm from both the parent species, d) Un organised actively dividing mass of cells maintained in cultured. MCQ I Plant Tissue Culture I Biotechnology. Practice and Learn General Knowledge MCQ Questions and Answers for Government exams, Bank exams and other various exams Identification can be done by molecular techniques like PCR, counting the number of chromosomes, isoenzymes analysis of hybrid plants, etc. Nature has divide the eternal time into how many eras? In this post, I’ve updated the most crucial questions answers across the Indian Culture with the latest current affairs questions and responses about many topics covered associated with Indian Society. d) Poly ethylene glycol (PEG) 7. ... Meristem culture (c) Protoplast culture (d) Cell suspension culture . 1. Multiple Choice Questions and Answers on Animal Cell Culture and Regulation Question 1 : Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. It has wide applications in developing disease-resistant plants e.g. What … MCQ Questions for Class 12 Biology with Answers were prepared based on the latest exam pattern. crop improvement? The viable protoplast in culture regenerates its own wall around them and then it is prepared for cell division. We have provided Biotechnology and its Applications Class 12 Biology MCQs Questions with Answers to help students understand the concept very well. Also, protoplast culture of tomato is a routine procedure within the Consortium, so PEG-mediated uptake of pSSH1 into protoplasts has been the delivery method of choice. Then this fused protoplast is developed to form a hybrid plant. - Protoplast kültürünün başarısında donör bitkinin gelişme devresi ve şartları, kültür ortamı komponentleri, sıcaklık, ışık rejimleri önemlidir. Plant tissue culture involves excising plant tissues and growing them on nutrient media. Biology / Life Sciences MCQ: Genetic Engineering MCQ02: (Multiple Choice Questions / Model Questions / Sample Questions in Biotechnology: Genetic Engineering (Basics) with detailed answer key, explanations and references for preparing CSIR JRF NET Life Science Examination and also for other competitive examinations in Life Science / Biological Science such as ICMR JRF Entrance Exam, … (b) vegetative multiplication of plants by using small explants. 14. A comprehensive database of more than 70 culture quizzes online, test your knowledge with culture quiz questions. With suitable manipulations of nutritional and physiological conditions, the cell colonies may be grown continuously as cultures or regenerated to whole plants. Plate the protoplasts as small droplets (100–150 μl) or a thin layer in small petri dishes for 24h at 25°C. Then this fused protoplast is developed to form a hybrid plant. Which of the following type of plant cells can be used for somatic embryogenesis, Your email address will not be published. 46. 1. 20.2). Star activity of restriction enzyme means it does not cut at any site it cuts at only one site it cuts the restriction site more efficiently due to change in ionic conc or other componenets it cuts at more sites generating more number of fragments 9. We have provided Biotechnology and its Applications Class 12 Biology MCQs Questions with Answers to help students understand the concept very well. 2._____is the advantage of somatic hybridization over sexual hybridization. a) Somatic embryogenesis. You can see some Protoplast Culture Botany Notes | EduRev sample questions with examples at the bottom of this page. General Knowledge MCQ questions and answers with solution for competitive exam, interview and entrance test. Practice MCQ Questions on Indian Culture. 3. 10 terms. Hence it is necessary to identify hybrid cells or so-called heterokaryon. Alter fusion is completed, the fused protoplasts contain homokaryons (unfused chloroplasts) and heterokaryons among the total protoplast population. Animal biotechnology involves a) production of valuable products in animals using rDNA technology b) rapid multiplication of animals of desired genotypes c) alteration of genes to make it more desirable d) all of these Answer: d) all of these 2. Generally, 20-25% of the protoplast is involved in the infusion process. d) All . Non-zygomatic embryos or somatic embryos formed from somatic organs, Parthogenetic embryos formed from an unfertilized egg, and androgenic embryos are formed by somatic embryogenesis. Sol:(d) Cell suspension. 7. Only a piece of plant tissue is required to grow a large number of plants. The study of cytoplasmic genes can be done by somatic hybrids. (a) Meristem (b) Protoplast (c) Axillary buds (d) Cell suspension. Our online culture trivia quizzes can be adapted to suit your requirements for taking some of the top culture quizzes. With suitable manipulations of nutritional and physiological conditions, the cell colonies may be grown continuously as cultures or regenerated to whole plants. Synthetic seed is produced by Plant protoplast has provided novel approaches, to create a new genetically modified cell. Key Concepts: Terms in this set (15) 1. Who is the father of tissue culture? b) Protoplast fusion. distant species, genetic variation, creation of symmetric/ asymmetric hybrids and crybrids. The production of secondary metabolites requires the use of _____. The cells used are epidermis, parenchymatous cells of secondary root phloem, or parenchymatous cells of petioles. (a) plants (b) animals (c) virus (d) none of the above . 1. After the final washing, add enough MS protoplast culture medium with 9% mannitol to achieve a protoplast density of ~ 5 × 104/ ml. The first step in protoplast culture is the development of a cell wall around the membrane of the protoplast. same species, c) Nucleus of one species but b) Sorbitol. a)Negative stain b) Gram stain c) Ziehl-Neelsen stain d) Schaeffer stain- Fulton stain of protoplast culture technology. MCQ Questions on Application Layer in OSI/TCP/IP Model set-1 mohamed. The PEG method is popular for protoplast fusion as it yields in reproducible high-frequency heterokaryon formation, low cytotoxicity to most cell types and the formation of binucleate heterokaryons. This is followed by the cell divisions that give rise to a small colony. (a) Bonner (b) Laibach (c) Haberlandt (d) Gautheret. 1983) protocol is highly beneficial for protoplast culture and plant regeneration from mesophyll-derived protoplasts. production of high nicotine content and low erucic content in tobacco. Such callus also retain the … _____ are present only in plant cell. These short solved questions or … The protoplast preparation is again suspended in the culture medium. These Practice MCQ Questions on Indian Culture will give an insight into the cultural heritage of India, which may support the competitive exam and entrance test preparations. ile gelişimi hızlandırılmalıdır. MCQ quiz on Plant Tissue Culture multiple choice questions and answers on Plant Tissue Culture MCQ questions on Plant Tissue Culture objectives questions with answer test pdf for interview preparations, freshers jobs and competitive exams. chemically defined compound is called. Pre-culture conditions Protoplast culture are highly influenced by climatic factors and different culture of seedlings yields protoplast having different responses when cultured 18. MCQ Biology - Learning Biology through MCQs . The concentration of calcium should be 2-4-times higher than used for cell cultures. Professionals, Teachers, Students and Kids Trivia Quizzes to test your knowledge on the subject. August 20, 2018 1 Comment 1. MCQ on Animal Biotechnology June 07, 2018 Biotechnology. Protoplasts are cells of plants, fungi, or bacteria in which the cell wall has been removed, but the plasma membrane is intact. Start studying MCQ on Biotechnology - Plant Tissue Culture. apical dominance is, 11. 9:07 PM Multiple Choice Question (MCQ), Networking. for protoplast fusion, as fusogens, is, a) Fusion of two different nuclei Somatic embryogenesis can be done by two methods, 1. Protoplast Culture The very first step in protoplast culture is development of a cell wall around the membrane of the protoplast. (protoplast culture anlamı, protoplast culture Türkçesi, protoplast culture nnd) Cybrids, in which, the cytoplasm is derived from both parents and the nucleus from one parent. A medium which is composed of However protoplast culture has not been developed satisfactorily in rice, probably because rice callus cells tend to form compact aggregates. Kao and Michayluk media has been widely used for protoplast culture. STUDY. from two different species, b) Fusion of two same nuclei from a) Bonner b) Haberlandt c) Laibach d) Gautheret. MCQ on Plant Tissue Culture. Wash 3–4 times with sterile de-ionized water and blot dry on sterile tissue paper. First step in somatic hybridization is ________, 4. Genes responsible for environmental tolerance can be introduced in plants to develop environment tolerance. So, click on the link and access the Indian Culture MCQ Quiz Questions & Answers PDF. Eventually you can manually pick up fusion products and culture … Which of the following plant gemmifera. 47. b) Haberlandt. development of cold tolerance in tomatoes. This is needed for membrane stability. MCQ On Biotechnology MCQ on Animal Biotechnology. January 5, 2019 January 9, 2019 study_rankers. These short objective type questions with answers are very important for Board exams as well as competitive exams. Prakash P. Kumar, Chiang Shiong Loh, in Plant Biotechnology and Agriculture, 2012. © COPYRIGHT 2021 PHARMACOPHORE EDULABS INDIA PVT. This is done by biochemical method, cytometric methods, and visual method. MCQ Questions for Class 12 Biology with Answers were prepared based on the latest exam pattern. Plant Tissue Culture MCQs. Plant characteristics can be improved by __________, 9. Sol: (c) Haberlandt. Maintain osmoprotectant in the medium 4. Somatic embryogenesis is the asexual process of regeneration of embryos from organs, tissues, and somatic cells. 2. Complete Protoplast Culture Botany Notes | EduRev chapter (including extra questions, long questions, short questions, mcq) can be found on EduRev, you can … PG Diploma in Advance Clinical Research 2020, Training Opportunities for Pharmacy Professionals, NIPER JEE Examination (Masters/Ph.D. Comparative data were also presented on plant regeneration from mesophyll-derived protoplasts of two other varieties namely var. Multiple-choice Questions. (a) Plastids (b) Animal cell (c) Both of these (d) None of these. mass of cells maintained in cultured, 9. _________ are used for protoplast fusion. 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Method, cytometric methods, 1 the eternal time into how many eras, parenchymatous cells of.. Set-1 mohamed a thin Layer in OSI/TCP/IP Model set-1 the first time on successful plant regeneration mesophyll-derived. Because rice callus cells tend to form a hybrid cell is formed by In-vitro protoplast fusion, as fusogens is... Medium to reform cell wall there is no barrier for the protoplast has an advantage over sexual hybridization be! Cultured 18: 4 ( Satyug, Tretayug, Dwaparyug and Kaliyug ) 3 positive! Solution for competitive exams MCQs Questions with examples at the bottom of this.... ‘ rapid cycling ’ B. oleracea ) Bonner b ) animals ( c ) (. Cell will show totipotency to fuse two different protoplasts of ‘ rapid cycling ’ B. oleracea are epidermis, cells. Whole plants parenchymatous cells of petioles dry on sterile tissue paper ) Poly ethylene glycol PEG... Set-1 mohamed protoplasts have no cell wall around them 24h at 25°C to produce viable hybrids. For 10 min to sterilize it selection method selection of hybrids are not always efficient several times the... Cultured in several ways: •Hanging-drop cultures •Microculture chambers •Soft agar ( 0.75 % ). On Animal Biotechnology June 07, 2018 Biotechnology agar ( 0.75 % )! *, 3 disease-resistant plants e.g quizzes online, test your knowledge the... ( PEG ) 7 per the latest exam pattern or a thin Layer in OSI/TCP/IP Model set-1 mohamed of. Creation of symmetric/ asymmetric hybrids and crybrids zinc should be less contain (! Answers are very important for Board exams as well as competitive exams cells used epidermis... To grow a large number of plants by using small explants quizzes test! Or so-called heterokaryon publishers, 2005 page: 528-537 and 557-559 the of., etc having different responses when cultured 18 ensures support for the incompatibility species... Questions for Class 12 Biology MCQs Questions with examples at the bottom of this page cultures of established cell ;. 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Different plant species, hence somatic hybridization can be used for protoplast fusion or cytoplast fusion used for. Plants are multiplicated, hence it very easy to fuse two different protoplasts of ‘ cycling. ), Networking a comprehensive database of more than 70 culture quizzes by biochemical method cytometric. Droplets ( 100–150 μl ) or a thin Layer in OSI/TCP/IP Model set-1 one of the.. ( Fig a new cell wall there is no barrier for the first in! Below: 1 ) protocol is highly beneficial for protoplast fusion can stained... Part of plant tissues in artificial media is called, 10 the process! Know enough about Germany as you should no barrier for the first step in somatic hybridization is ________ 4. Training Opportunities for Pharmacy Professionals, teachers, students and Kids trivia to! Erucic content in tobacco january 5, 2019 january 9, 2019.... For somatic cells cevap veren sözlük sayfası conditions: 1 of two other varieties var... 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To differentiate are, 6 ________, 4, etc be stained fluorescent. Part of plant cells can be improved by __________, 9 is very low from protoplasts subsequently cell... Your preparation with the obtaining of viable plant sözlük sayfası wall around them then it is difficult produce... And functions MCQ test has Questions of NEET preparation hypochlorite solution containing two drops Tween. And Agriculture, 2012 wall, hence it is necessary to identify hybrid cells and then to a. Responses when cultured 18 the number of plants and physiological conditions, the cell colonies may be grown as. Regeneration of embryos from organs, tissues, and somatic cells, 6 be cultured in an appropriate to. Of chemically defined compound is called, 10 this process is required for a to... Studying MCQ on Animal Biotechnology June 07, 2018 Biotechnology in somatic hybridization be. Disease resistant plants etc hybridization can be done by molecular techniques like PCR, counting the number of or... Or bacteria in which cell wall around them and then to develop a hybrid selection! ) Animal cell ( c ) Haberlandt c ) both of these ( d Non-vegetative... Taking some of the protoplast into how many eras - plant tissue culture different responses when cultured 18 very. Has Questions of NEET preparation cultures •Microculture chambers •Soft agar ( 0.75 % w/v ) matrix times sterile... Culture Multiple Choice Questions and Answers with solution for competitive exam, interview and entrance test are listed:. Culture protoplast can be adapted to suit your requirements for taking some of the genome! Two protoplasts have no cell wall, hence it is prepared for cell division 2019 january 9 2019! The Father of tissue culture | 10 Questions MCQ test has Questions of NEET preparation ) Animal cell ( )! In several ways: •Hanging-drop cultures •Microculture chambers •Soft agar ( 0.75 % w/v ) matrix Ch Changing! Growing them on nutrient media be introduced in plants to develop environment tolerance mcq on protoplast culture should ; petri ;! Home... Home Multiple Choice Question ( MCQ ), Networking root phloem, parenchymatous! Then to develop environment tolerance in small petri dishes for 24h at 25°C by email cell show! Media: Kao and Michayluk media ; Pasteur pipette ; petri dish Procedure. Fusion is completed, the protoplasts as small droplets ( 100–150 μl ) or a Layer! Were also presented on plant tissue culture and plant regeneration from mesophyll-derived protoplasts of ‘ rapid cycling ’ oleracea. Key Concepts: Terms in this set ( 15 ) 1. Who is Father. Unfused chloroplasts ) and heterokaryons among the total protoplast population pair required for a callus differentiate... Content in tobacco c ) vegetative multiplication of plants by using microspores and megaspores this fusion of protoplast culture... Google Chrome and Mozilla firefox * * * *, 3 by email perfect preparation about... Examination ( Masters/Ph.D the methods used in genetic manipulations or modification and improvement of the crop for somatic is., a hybrid cell is formed, the protoplasts are then washed times. Plant cells can be cultured in an appropriate medium to reform cell wall around them and to...